Description

Nonomuraea mesophila (me. so ′ phi. la Gr. n. mesos middle; Gr. fem. adj. philos loving; N. L. fem. adj. mesophila middle temperature loving). Aerobic, Gram-stain-positive, non-motile actinobacterium that forms extensively branched substrate and aerial mycelia. Aerial hyphae differentiate into spore chains with smooth surfaces. Good growth occurs on ISP 2, ISP 4, modified Bennett’s and tryptic soy agar and moderate growth on Czapek’s, nutrient and ISP 5 – 7 agar. A range of substrate mycelial pigments are formed including cream, light brown, dark brown, reddish brown, reddish black or claret red ones. Melanoid pigments are not produced on the ISP 6 agar. Red coloured diffusible pigments are observed on modified Bennett’s agar. Grows from 20 – 37 ° C (optimum, 28 ° C), from pH 6.0 – 8.0 (optimum, pH 7.0) and in the presence of up to 5 % (w / v) NaCl. Hydrolyses aesculin, reduces nitrate, but does not produce H 2 S, does not hydrolyse allantoin, arbutin or urea. Degrades hypoxanthine, starch and Tweens (20, 40 and 80), but not adenine, casein, chitin, gelatin, guanine, xanthine or xylan. Adonitol, D-arabinose, D-fructose, raffinose, cellobiose, D-galactose, melezitose, melibiose, dextran, D-glucose, inulin, lactose, D-maltose, D-mannitol, sucrose and D-xylose are used as sole carbon and energy sources but not D-sorbitol, D-mannose, dextrin, L-sorbose, L-arabinose, L-rhamnose, L-glutamine, myo-inositol, sodium succinate or xylitol. L-Alanine, L-arginine, L-asparagine, L-cysteine, glycine, L-hydroxyproline, α-isoleucine, L-methionine, L-phenylalanine, L-proline, L-serine, L-tyrosine and L-valine are utilized as sole nitrogen sources, but not L-histidine. The predominant menaquinones are MK- 9 (H 4) and MK- 9 (H 6). The polar lipid profile include diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids, an unidentified glycophospholipid, four unidentified phospholipids and an unidentified lipid. Whole-cell hydrolysates include meso - A 2 pm, glucose, mannose, madurose and ribose. The major fatty acids are C 16: 0, iso-C 16: 0 and C 17: 0 10 - methyl. The DNA G + C content of strain 6 K 102 T is 70.8 mol % and its genome size 10.14 Mbp.

Common Names

No common names available.

Taxonomic Hierarchy

Kingdom: Bacteria

Phylum: Actinobacteriota

Class: Actinomycetia

Order: Streptosporangiales

Family: Streptosporangiaceae

Genus: Nonomuraea

Species: Nonomuraea mesophila